Biocatalytic Applications and Mechanistic Insights of Deoxypodophyllotoxin Synthase
Abstract
-dimethoxy analogue of (+)-hydroxy-yatein indicate antarafacial C-C bond formation during the cyclization reaction and remain consistent with cation-mediated cyclization. A crystallographic study of DPS bound with vanadium(IV) oxide, succinate, and (-)-hydroxy-yatein suggests a subtle interplay of steric interactions between the substrate and the active site that can alter the course of the DPS-catalyzed reaction and thus cyclization versus hydroxylation. Finally, an efficient chemoenzymatic approach to (-)-podophyllotoxin is described that relies only on freeze-dried whole cells after DPS overexpression.
Fields of Study & Subjects
Plant-derived Lignans Synthesis and Bioactivity
Plant Gene Expression Analysis
Bioactive natural compounds
Publication details
- Journal / source
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Volume
- 148
- Issue
- 15
- Pages
- 16394–16403
- Publication date
- 7 April 2026
- Language
- EN