Extending Serum IgG1 Antibody Repertoire Coverage Using DIA-PTCR
Abstract
Detecting more and less abundant antibody clones in serum by LC-MS-based Fab profiling is challenging as all molecules are alike, leading to extreme spectral congestion following electrospray ionization. To reduce spectral congestion, we implemented gas-phase fractionation using proton transfer charge reduction in a data-independent analysis manner (DIA-PTCR). Employing just five isolation windows over a limited m / z region was found to be sufficient to cover the total Fab distribution. This gas-phase fractionation, when also combined with reduced noise filtering, increased the number of clones detected within a single run from 1043 to 2063 (+100%). Importantly, quantitative data on antibody abundances could be retained as required for repertoire profiling. We conclude that DIA-PTCR is beneficial for LC-MS-based antibody repertoire profiling.
Fields of Study & Subjects
Monoclonal and Polyclonal Antibodies Research
Advanced Proteomics Techniques and Applications
Advanced Biosensing Techniques and Applications
Publication details
- Journal / source
- Journal of the American Society for Mass Spectrometry
- Publisher
- American Chemical Society (ACS)
- Volume
- 37
- Issue
- 6
- Pages
- 1423–1429
- Publication date
- 11 May 2026
- Language
- EN