UNCOVERseq enables sensitive and controlled gene editing off-target nomination across CRISPR-Cas modalities and systems

Nature Communications 2026 Open access

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Authors: Kyle J. Kinney, Kun Jia, He Zhang, Ellen Schmaljohn, Thomas F. Osborne, Bernice Thommandru, Karthik Murugan, Andrea Sánchez Peña, Sean West, Shengyao Chen, Roshani Codipilly, Morgan Sturgeon
+7 more Rolf Turk, Matthew McNeill, Mark A. Behlke, Ashley M. Jacobi, M. Kyle Cromer, Garrett Rettig, Gavin L. Kurgan
DOI: 10.1038/s41467-026-74623-7
Cited by: 4
References: 52

Abstract

Abstract The rapid development of CRISPR-Cas gene editing technologies has revolutionized genetic medicine, offering unprecedented precision and potential for treating a wide array of genetic disorders. However, assessing the risks of unintended gene editing effects remains critical, and is complicated by new editing modalities and unclear analytical guidelines. We present UNCOVERseq (Unbiased Nomination of CRISPR Off-target Variants using Enhanced RhPCR), an improved in cellulo off-target nomination workflow designed to sensitively nominate off-target sites (

Fields of Study & Subjects

CRISPR and Genetic Engineering

Publication details

Journal / source
Nature Communications
Publisher
Springer Science and Business Media LLC
Volume
17
Issue
1
Publication date
11 August 2026
Language
EN