CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

Nature Communications 2026 Open access

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Authors: Moises Di Sante, Melissa Pezzotti, Julius Zimmermann, Alessandro Enrico, J.R. Deschamps, Elisa Balmas, Silvia Becca, Eloisa Torchia, Giulia Ballio, Samantha Solito, Martina Sarchi, Alessandro Reali
+3 more Alessandro Bertero, Florian Jug, Francesco S. Pasqualini
DOI: 10.1038/s41467-026-76144-9
Cited by: 7
References: 27

Abstract

Abstract Cell cycle progression, migration, and proliferation shape development and regeneration, but simultaneous live-cell imaging remains challenging as conventional fluorescent cell cycle indicators (FUCCI) monopolize the green and red channels used by most structural and functional biosensors. To overcome this, we integrated a spectrally re-engineered FUCCI variant, open-source analysis software, and four-color human stem cell reporter lines into CALIPERS: a method for Cell-cycle-Aware Live-cell Imaging in Phenotyping and Regeneration Studies.

Fields of Study & Subjects

Cell Image Analysis Techniques

Publication details

Journal / source
Nature Communications
Publisher
Springer Science and Business Media LLC
Volume
17
Issue
1
Publication date
1 August 2026
Language
EN