Disequilibrium between chloroplast proton motive force and ATP levels in Arabidopsis
Abstract
Abstract Current dogma holds that CO 2 fixation by photosynthesis requires additional ATP production via PGR5-dependent cyclic electron transfer (PGR5-CET) to augment the NADPH and ATP produced by linear electron transfer (LET). Here we use the MgATP 2− fluorescent biosensor ATeam1.03-nD/nA and find that while proton motive force (pmf) in the Arabidopsis pgr5CAS mutant is only 50-75% of that of the wild-type, MgATP 2− levels are unchanged. These data demonstrate that disequilibrium exists between pmf and MgATP 2− concentration in vivo, indicating that PGR5-CET is not required for ATP augmentation.
Fields of Study & Subjects
Photosynthetic Processes and Mechanisms
ATP Synthase and ATPases Research
Mitochondrial Function and Pathology
Publication details
- Journal / source
- Nature Plants
- Publisher
- Springer Science and Business Media LLC
- Volume
- 12
- Issue
- 9
- Pages
- 1679–1687
- Publication date
- 17 August 2026
- Language
- EN