Chromosome-level genome assembly of the Siniperca obscura

Siniperca obscura is an economically valuable and ecologically significant species in China, yet the lack of comprehensive genomic resources has hindered genetic studies and breeding efforts. In this study, we present a high-quality, chromosome-level genome assembly for S. obscura, generated by integrating PacBio HiFi long-read sequencing with Hi-C scaffolding. The final assembly spans 734.12 Mb, with 99.85% of the assembled bases anchored and oriented onto 24 chromosomes. It achieves a contig N50 of 24.59 Mb and a scaffold N50 of 30.62 Mb, with high genome completeness further demonstrated by a BUSCO score of 99.37%. We predicted 23,225 protein-coding genes, with a 99.12% BUSCO completeness, and 98.50% of the genes were functionally annotated. Approximately 30.54% of the genome sequences were classified as repeat elements. This high-quality reference genome provides a valuable resource for advancing molecular breeding, comparative genomics, and evolutionary studies of S. obscura and closely related species.