LowLoad-qPCR as a novel clinical strategy for detecting low-load bacteremia

Scientific Reports 2026 Open access

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Authors: Paula Gómez Estévez, José Miguel Cisneros, José Antonio Lepe, Guillermo Martín-Gutiérrez, José Manuel Ortiz de la Rosa
DOI: 10.1038/s41598-025-34230-w
Cited by: 0
References: 18

Abstract

The primary obstacle in detecting bloodstream infections is their often extremely low bacterial concentration. To accurately detect such infections, we developed a quantitative PCR assay, named LowLoad-qPCR, based on the combination of increased input DNA by random priming amplification method and the use of multiple probes sharing a single fluorophore. Here, we detail the key improvements over conventional qPCR and evaluate its performance using blood samples.

Fields of Study & Subjects

Bacterial Identification and Susceptibility Testing Biosensors and Analytical Detection Molecular Biology Techniques and Applications

Publication details

Journal / source
Scientific Reports
Volume
16
Issue
1
Pages
4163–4163
Publication date
3 January 2026
Language
EN