Rapid clearance of bacteria from maternal bloodstream after delivery in pregnancies complicated by preterm pre-labor rupture of the membranes

Preterm pre-labor rupture of membranes (PPROM) increases maternal and neonatal sepsis risk, yet its association with maternal microbial translocation and systemic inflammation remains unclear. We investigated whether PPROM is linked to microbial DNA in maternal blood (MB) and inflammatory responses around delivery. In 66 PPROM patients (median GA: 32±1 weeks), MB was collected pre-delivery and within 1 hour postpartum. Fetal membranes (FM) and placental tissues were sampled immediately after delivery. Bacterial load and diversity were analyzed via 16S rDNA qPCR and sequencing. Maternal cytokines were quantified by multiplex immunoassay, and fetal inflammatory exposure (Triple I) assessed using histological chorioamnionitis (HCA), cord blood haptoglobin, and IL-6. Bacterial DNA was detected in maternal blood (MB) pre- and post-delivery, with a significant postpartum decline (p=0.004). FM carried higher bacterial load and biodiversity than placenta (p