AAV.PHP.eB-based strategies for precise modulation of α7 nicotinic acetylcholine receptor in neurons and astrocytes in the adult mouse brain
Abstract
Precise control of the α7 nicotinic acetylcholine receptor (α7-nAChR) in specific brain cell types is crucial for understanding its role in neural circuit function and disease. Here, we introduce a set of AAV.PHP.eB-based gene delivery vectors optimized for cell-type-specific downregulation or overexpression of α7-nAChR in the adult mouse brain. Using promoters for neuronal or astrocytic targeting, we achieved efficient cell-type modulation of α7-nAChR after intra-hippocampal administration. These vectors provide a versatile platform for investigating α7-nAChR-dependent mechanisms in vivo and advancing the preclinical development of targeted interventions for neurological disorders characterized by receptor dysregulation.
Fields of Study & Subjects
Nicotinic Acetylcholine Receptors Study
Vagus Nerve Stimulation Research
Receptor Mechanisms and Signaling
Publication details
- Journal / source
- Scientific Reports
- Publisher
- Springer Science and Business Media LLC
- Volume
- 16
- Issue
- 1
- Publication date
- 1 April 2026
- Language
- EN