DNA methylation modulation by 5-Azacytidine regulates EMT markers and reduces invasiveness in docetaxel-resistant prostate cancer cells

Abstract Background: Metastatic castration-resistant prostate cancer (mCRPC) is associated with poor prognosis and high mortality, largely due to the emergence of androgen and chemotherapy resistance. Resistance to docetaxel occurs in nearly 50% of patients and remains a major clinical challenge. DNA methylation is known to contribute to therapy resistance through gene silencing and reprogramming of cellular pathways. This study investigated whether global DNA hypomethylation achieved through DNMT1 inhibition could reduce cell invasion and motility in docetaxel-resistant (DR) prostate cancer (PCa) cells. Results: Parental and DR PCa cell lines (22Rv1, PC3, and DU145) were treated with 5-Azacytidine (5-AZA: 0.5–16 µM) for 72 hrs. Treatment with 5-AZA reduced cell viability in a dose-dependent manner in all cell models except DU-145DR cells. DNMT1 inhibition was confirmed in all cell lines, while re-expression of GSTP1, indicating successful DNA hypomethylation, was observed in DU145-DR and PC3/PC3-DR cells. No changes were observed in androgen receptor (AR), AR variant 7 (AR-V7), or MDR1 protein expression. Invasion capacity was significantly reduced in DU145-DR (1 µM: p