Evaluation of ToF-SIMS imaging for semi-quantitative mapping of BODIPY-labeled fibronectin surface gradients

The Analyst 2026 Open access

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Authors: Chao Liu, J. Kim, Douglas H. Wu, Radhika P. Atit, Rodrigo A. Somoza, Samuel E. Senyo
DOI: 10.1039/d5an00962f
Cited by: 0
References: 35

Abstract

or higher. Semi-quantitative measurements using imaging mass spectrometry confirmed a graded distribution of fibronectin within the physiologically relevant "haptotaxis zone", with detection sensitivity exceeding the limits of standard fluorescent microscopy. These results establish ToF-SIMS as a valid method for detecting surrogate-labeled ligand gradients, providing a reliable approach for future quantitative ligand measurements.

Fields of Study & Subjects

Mass Spectrometry Techniques and Applications Ion-surface interactions and analysis Advanced Proteomics Techniques and Applications

Publication details

Journal / source
The Analyst
Volume
151
Issue
4
Pages
1118–1129
Publication date
1 January 2026
Language
EN