CRISPR/Cas13a-induced self-priming cyclic amplification enables liquid biopsy of exosomal circular RNA in non-small cell lung cancer
Abstract
-cleavage activity of Cas13a and the high amplification efficiency of the self-priming cyclic reaction, the assay achieves sensitive detection of circRNA at concentrations as low as 564 aM within 90 min. In addition, the proposed method has been successfully applied for the analysis of exosomal hsa_circ_0003026 expression level in normal samples and NSCLC samples and demonstrated the potential of exosomal hsa_circ_0003026 in regulating the pathological progression. Owing to the high specificity of Cas13a, the proposed method can be directly applied to detect circRNA in complex biological samples without prior isolation of corresponding linear RNAs.
Fields of Study & Subjects
Circular RNAs in diseases
Extracellular vesicles in disease
Ferroptosis and cancer prognosis
Publication details
- Journal / source
- The Analyst
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 151
- Issue
- 5
- Pages
- 1413–1419
- Publication date
- 1 January 2026
- Language
- EN