Rapid and colorimetric assay for the detection of S. pneumoniae based on hydrogen peroxide release and analysis using color image processing

The Analyst 2026 Open access

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Authors: Cagla Celik Yoldas, Nimet Temur, Nilay Ildız, Pinar Sagiroglu, Mustafa Altay Atalay, Ali Osman Yılmaz, Ersen Göktürk, İsmail Öçsoy
DOI: 10.1039/d5an01356a
Cited by: 1
References: 43

Abstract

, which induces a distinct color shift to gray. We systematically evaluated the performance of this assay in various matrices, including growth medium, saline solution, and artificial cerebrospinal fluid (aCSF). Bacterial suspensions of 1.0 and 1.5 McFarland were colorimetrically detected within 90 and 30 minutes, respectively, through naked-eye observation and Delta-E analysis. This study demonstrates a highly stable, cost-effective, and easy-to-prepare diagnostic tool that offers significant advantages over traditional methods for point-of-care testing.

Fields of Study & Subjects

Biosensors and Analytical Detection Advanced Chemical Sensor Technologies Pneumonia and Respiratory Infections

Publication details

Journal / source
The Analyst
Publisher
Royal Society of Chemistry (RSC)
Volume
151
Issue
6
Pages
1669–1678
Publication date
1 January 2026
Language
EN