CRISPR/Cas12a platform activated by a protospacer adjacent motif-engineered DNA circuit for specific target sensing
Abstract
for uracil-DNA glycosylase (UDG), and 0.12 fM for acetamiprid (ACE). This approach exemplifies a two-stage signal amplification mechanism, achieving improved sensitivity relative to either the CRISPR/Cas12a system or the DNA circuits alone. Moreover, quantitative assays for these targets were successfully conducted in real samples, suggesting the practical applicability of the proposed method. This research establishes a versatile sensing platform for various targets, which holds significant promise for advancements in molecular diagnostics, food safety assessment, and environmental monitoring.
Fields of Study & Subjects
Advanced biosensing and bioanalysis techniques
CRISPR and Genetic Engineering
Biosensors and Analytical Detection
Publication details
- Journal / source
- Analytical Methods
- Volume
- 18
- Issue
- 7
- Pages
- 1397–1405
- Publication date
- 1 January 2026
- Language
- EN