Adapting aqueous biphasic systems to solid-phase extraction for bioanalytical applications: high-abundance protein depletion and prostate-specific antigen extraction from human serum
Abstract
Aqueous biphasic systems are investigated here, from standard liquid-phase to solid-phase extraction configurations for human serum pretreatment. Using prostate-specific antigen as a model biomarker, this strategy achieves >90% depletion of high-abundance proteins and demonstrates strong potential for bioanalytical applications compared to conventional precipitation methods.
Fields of Study & Subjects
Chemical and Physical Properties in Aqueous Solutions
Analytical chemistry methods development
Advanced Proteomics Techniques and Applications
Publication details
- Journal / source
- Analytical Methods
- Volume
- 18
- Issue
- 11
- Pages
- 2188–2192
- Publication date
- 1 January 2026
- Language
- EN