Plasmonic fluorescent Ag/C-based nanohybrids for treatment of chemically-induced cutaneous burns

Chemical burns are a significant concern in Ukraine, with a growing interest in new treatments. Nanohybrids based on Ag nanoparticles and carbon dots (Ag/C NHs) due to their anti-inflammatory, antioxidant, and antibacterial properties, have shown promise for wound healing. This study aimed to evaluate the efficacy of Ag/C NHs in promoting healing of both acidic- and alkali-induced burns in a rat model. The results indicated that rats with acid- and alkali-induced skin burns exhibited distinct healing patterns and systemic responses, with Ag/C197 sample accelerating early wound closure in acidic burns despite a mild proinflammatory profile, while Ag/C195 sample promoted later-stage healing and demonstrated anti-inflammatory effects, particularly in alkali burns. Systemic inflammation was evident in both burn types, characterized by neutrophilia and lymphocyte suppression, which was mitigated by Ag/C195. Both Ag-based formulations increased monocyte counts, suggesting enhanced tissue repair. Additionally, burns induced mild anemia that was not fully prevented by treatment with Ag/C NHs, although partial hemoglobin recovery was observed by day 14. Overall, our findings support the therapeutic potential of Ag/C NHs (particularly Ag/C195 version), in managing chemical burn injuries.