Optical control of Cas9 activity through visible-light cleavable crRNAs

RSC Chemical Biology 2026 Open access

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Authors: Vanessa Hanff, Stepan Jerabek, Kim A Langer, Robin Klimek, Janik Kaufmann, Jimi Kim, Dieter Egli, Alexander Heckel
DOI: 10.1039/d5cb00294j
Cited by: 1
References: 49

Abstract

Cas9 assay developed in this work. By using visible light, our approach will enable precise downregulation of Cas9 activity in terms of location and timing, thereby enabling non-invasive and harm free control over genome editing for safer application.

Fields of Study & Subjects

Photochromic and Fluorescence Chemistry Retinal Development and Disorders CRISPR and Genetic Engineering

Publication details

Journal / source
RSC Chemical Biology
Publisher
Royal Society of Chemistry (RSC)
Volume
7
Issue
4
Pages
649–655
Publication date
1 January 2026
Language
EN