Development of a transcription factor-based biosensor strain for reporting α-terpineol production via the alcohol-dependent hemiterpene pathway in Escherichia coli
Abstract
by coupling it to an artificial alcohol-dependent hemiterpene (ADH) pathway and downstream modules expressing GPPS and α-terpineol synthase. The integrated biosensor-production system effectively distinguishes the complete biosynthetic pathway from deletions and reports intracellular titers consistent with GC-MS quantification. Together, these results provide the first biosensor for monocyclic monoterpene alcohols and establish a compact, modular framework for high-throughput screening and pathway optimization. This platform sets the stage for accelerating the discovery, engineering, and scalable bioproduction of valuable isoprenoids and other terpene-derived natural products.
Fields of Study & Subjects
Plant biochemistry and biosynthesis
Microbial Metabolic Engineering and Bioproduction
Microbial Natural Products and Biosynthesis
Publication details
- Journal / source
- RSC Chemical Biology
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 7
- Issue
- 4
- Pages
- 695–707
- Publication date
- 1 January 2026
- Language
- EN