Enzyme cascade reactions encapsulated in a liposome compartment with size-limited molecular transport
Abstract
We designed a liposome-coated DNA origami compartment to encapsulate a defined number of cascade enzymes with retained cofactors. Confinement enhanced cascade reaction efficiency particularly at low cofactor levels. Efficiency gains plateaued at higher NADH concentrations, indicating that intermediate diffusion and enzyme spatial organization influence cascade performance in confined environments.
Fields of Study & Subjects
Advanced biosensing and bioanalysis techniques
Supramolecular Self-Assembly in Materials
DNA and Nucleic Acid Chemistry
Publication details
- Journal / source
- Chemical Communications
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 62
- Issue
- 4
- Pages
- 1145–1148
- Publication date
- 1 January 2026
- Language
- EN