Enzymatic activation complex formation induced by crowding stress
Abstract
. Our results indicate that macromolecules significantly impact the association, dissociation, folding, and unfolding of protein molecules, which may result in several pathways in protein signaling and function in the human body. We have demonstrated that chemical pathways do not solely govern the formation of the CaM-eNOS complex, but that macromolecular crowding also plays a significant role in the formation of an active CaM-eNOS complex. Our results suggest that CaM signaling and NOS functions can be regulated with the combined action of chemical-induced and force-induced pathways, providing new insights into the force-induced pathways for the NOS enzymatic reactions.
Fields of Study & Subjects
Protein Structure and Dynamics
Nitric Oxide and Endothelin Effects
Protein Kinase Regulation and GTPase Signaling
Publication details
- Journal / source
- Physical Chemistry Chemical Physics
- Volume
- 28
- Issue
- 8
- Pages
- 5505–5515
- Publication date
- 1 January 2026
- Language
- EN