Mechanistic insights into the design of fluorogenic molecules for wash-free biological applications

Chemical Society Reviews 2026 Open access

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Authors: Rongrong Huang, Syed Ali Abbas Abedi, Guanyu Jiang, Chao Wang, Lingya Peng, Tianruo Shen, Nabil Mroweh, Yu Fang, Xiaogang Liu
DOI: 10.1039/d5cs01519g
Cited by: 12
References: 269

Abstract

fluorophore formation, twisted intramolecular charge shuttle, and conical intersections. By linking photophysical mechanisms to molecular design principles and imaging performance, this review aims to guide the development of next-generation fluorogenic probes for high-contrast, real-time, and sustained imaging across molecular, cellular, and organismal scales.

Fields of Study & Subjects

Click Chemistry and Applications Advanced Fluorescence Microscopy Techniques Luminescence and Fluorescent Materials

Publication details

Journal / source
Chemical Society Reviews
Publisher
Royal Society of Chemistry (RSC)
Volume
55
Issue
7
Pages
3957–4030
Publication date
1 January 2026
Language
EN