The dietary polyphenol resveratrol attenuates high-fat diet-induced testicular dysfunction via modulating the gut microbiota–testis metabolic axis
Abstract
) and normalized testicular metabolites, including the antioxidant 1,4-dithiothreitol and the membrane-stabilizing heptadecanoic acid. Network pharmacology predicted key targets (TNF-α, IL-1β, PTGS2, HRAS, and the TCA cycle enzyme SDHB), which we validated experimentally. RSV enhanced mitochondrial TCA cycle activity (upregulating SDHB, ACO2, CS and MDH2), suppressed the NF-κB pathway, and promoted repair processes. Molecular docking confirmed stable binding of RSV to these targets. Collectively, this work elucidates the mechanism by which dietary RSV alleviates testicular impairment, thereby providing a mechanistic blueprint for its rational application as a functional food ingredient. This work translates molecular insights into a tangible strategy for developing targeted functional foods or dietary supplements to address diet-related male subfertility.
Fields of Study & Subjects
Sirtuins and Resveratrol in Medicine
Sperm and Testicular Function
PARP inhibition in cancer therapy
Publication details
- Journal / source
- Food & Function
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 17
- Issue
- 6
- Pages
- 2782–2799
- Publication date
- 1 January 2026
- Language
- EN