A high-throughput liver-kidney metabolic interaction chip for insights into the nephrotoxicity mechanisms of triptolide

Lab on a Chip 2026

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Authors: Siyu Liu, Yun Yang, Yifei Yang, Guangfei Wei, Liu Zhou, Jiawei Lin, Zheng Yuan, Yingfei Li, Zhe Wu, Ting Liu, Guozhuang Zhang
DOI: 10.1039/d5lc01051a
Cited by: 3
References: 56

Abstract

conditions. Notably, a TPL-induced elevation in urea was observed only in the HLKOC, demonstrating the superior sensitivity of the liver-kidney co-culture. Multi-omics analysis revealed that TPL induced distinct metabolic and transcriptional responses in the HLKOC, involving pathways related to linoleic acid metabolism and vesicle-mediated processes, and led to the significant downregulation of transport proteins cubilin and GLUT1. These findings highlight the advantages of the HLKOC over single-organ systems for drug toxicity assessment and provide new insights into the mechanisms of TPL-induced nephrotoxicity.

Fields of Study & Subjects

Natural Compounds in Disease Treatment Renal Transplantation Outcomes and Treatments 3D Printing in Biomedical Research

Publication details

Journal / source
Lab on a Chip
Publisher
Royal Society of Chemistry (RSC)
Volume
26
Issue
6
Pages
1830–1849
Publication date
1 January 2026
Language
EN