Multi-wavelength transparent microfluidic device for UV-visible illumination and X-ray scattering studies of photoactive systems
Abstract
isomerization of azobenzene and fluoro-azobenzene. X-ray transparency was verified through small-angle X-ray scattering (SAXS) measurements on hemoglobin and CO-ligated hemoglobin, both of which are sensitive to quaternary structural changes. These results confirm the suitability of the device for resolving protein structures and detecting subtle conformational changes of the type commonly encountered in photo-induced modulation. Initial proof-of-concept measurements demonstrate the feasibility of temperature-jump (T-jump) experiments, and the same architecture is readily extendable to time resolved pump-probe studies, providing a versatile platform for studying structural evolution in liquid samples using synchrotron SAXS.
Fields of Study & Subjects
Hemoglobin structure and function
Spectroscopy and Quantum Chemical Studies
Innovative Microfluidic and Catalytic Techniques Innovation
Publication details
- Journal / source
- Lab on a Chip
- Volume
- 26
- Issue
- 9
- Pages
- 2697–2708
- Publication date
- 1 January 2026
- Language
- EN