Nitrogen cavitation enables rapid and high-yield preparation of functional cell-membrane-derived vesicles

Nanoscale Advances 2026 Open access

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Authors: Changting Li, Dehua Luo, Can Peng, Qun Luo, Wenjing Xu, Jincheng Zhou, Wen Su, Wei Ting Wu, Yi-Feng Wang
DOI: 10.1039/d5na00855g
Cited by: 3
References: 48

Abstract

Cell-membrane-derived vesicles (CMDVs) are increasingly utilized for the delivery of bioactive molecules due to their retention of membrane proteins, low immunogenicity, and biocompatibility. However, existing preparation methods (such as homogenization, sonication, and extrusion) struggle to strike a balance between scalability and structural integrity. More importantly, these methods lack subcellular selectivity, often leading to contamination from nuclei, mitochondria, lysosomes, and peroxisomes, which compromises the purity of the vesicles. Here, we present an optimized nitrogen cavitation-based workflow for the CMDV preparation within

Fields of Study & Subjects

RNA Interference and Gene Delivery Lipid Membrane Structure and Behavior Extracellular vesicles in disease

Publication details

Journal / source
Nanoscale Advances
Publisher
Royal Society of Chemistry (RSC)
Volume
8
Issue
6
Pages
2021–2030
Publication date
1 January 2026
Language
EN