Surface-enhanced stimulated Raman scattering and fluorescence probing of plasmonic nanoparticles in cellular environments: insights into their spatial distribution and aggregation
Abstract
, supported by scanning electron microscopy, was employed to examine the nanoparticle distribution within single cells, revealing the formation of "hot spots" due to nanoparticle agglomeration. This study underscores the limitations of using GNB for detailed cell imaging and metabolic investigations solely based on either SE-SRS or fluorescence imaging, which is due to the inconsistency of data obtained from either method alone. In contrast, the combined SE-SRS-fluorescence approach revealed detailed information on nanoparticle distribution and clustering within cellular environments, as well as the differentiation of "hot spots", providing valuable insights into nanoparticle uptake and possible applications in optical diagnostics and molecular biology.
Fields of Study & Subjects
Gold and Silver Nanoparticles Synthesis and Applications
Spectroscopy Techniques in Biomedical and Chemical Research
Nanoparticles: synthesis and applications
Publication details
- Journal / source
- Nanoscale Advances
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 8
- Issue
- 7
- Pages
- 2220–2232
- Publication date
- 1 January 2026
- Language
- EN