A pH-stable dynamic DNA nanomachine with controllable conformational switching

Nanoscale Advances 2026 Open access

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Authors: Lixuan Lin, Kaiyi Liang, Xiuli Gao, Jiang Li, Linjie Guo, Kai Jiao, Lihua Wang
DOI: 10.1039/d5na01166c
Cited by: 1
References: 27

Abstract

The heterogeneity of physiological pH poses a major challenge to the stability and function of dynamic DNA nanomachines. Here, we systematically evaluate the performance of a six-helix DNA nanomachine (6H-NNM) as a model system. It maintains reversible, strand-displacement-driven conformational switching across pH 5-9 (>81% intact after 8 h) and remains functional under lysosome-mimicking acidic conditions. Our study provides an evaluation framework and performance benchmark for dynamic DNA devices in complex biological settings.

Fields of Study & Subjects

Advanced biosensing and bioanalysis techniques DNA and Biological Computing DNA and Nucleic Acid Chemistry

Publication details

Journal / source
Nanoscale Advances
Publisher
Royal Society of Chemistry (RSC)
Volume
8
Issue
7
Pages
2215–2219
Publication date
1 January 2026
Language
EN