RNA nanostructures based on three-letter coding with non-canonical base pairs
Abstract
Synthetic RNA nanostructures are typically composed of four nucleotides (A, U, G, and C) following a canonical base pairing rule (A-U/G-C). G·U wobble pairs are commonly employed in many RNA nanostructures, but other non-canonical base pairing remains underexplored. In this work, we design RNA nanostructures with only three nucleotides instead of four. Besides Watson-Crick G-C base pairs, we incorporate A·C non-canonical base pairs into this three-letter coding scheme and allow selective nanostructure assembly from mixed DNA templates. With the new paradigm, we produce a variety of RNA nanostructures, further expanding the possibilities of rational molecular design.
Fields of Study & Subjects
RNA and protein synthesis mechanisms
DNA and Nucleic Acid Chemistry
Advanced biosensing and bioanalysis techniques
Publication details
- Journal / source
- Nanoscale Horizons
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 11
- Issue
- 4
- Pages
- 1048–1052
- Publication date
- 1 January 2026
- Language
- EN