Nanoscale protonation limits and charge density in polymer films govern the activity of immobilized LacZ under acid stress
Abstract
SpyCatcher/SpyTag binding). Directed immobilization preserved higher free LacZ activity than random immobilization, but the protonation-dependent protection trend remained consistent for both strategies. Together, these results identify protonation depth and charge density as orthogonal, tunable design parameters and establish a thickness regime that maximizes protection without unnecessary film growth.
Fields of Study & Subjects
biodegradable polymer synthesis and properties
Polymer Surface Interaction Studies
Enzyme Catalysis and Immobilization
Publication details
- Journal / source
- Nanoscale
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 18
- Issue
- 26
- Pages
- 13769–13780
- Publication date
- 1 January 2026
- Language
- EN