A synergistic dual-mode biosensor for methotrexate detection leveraging FRET and Ag NP-based UV absorption
Abstract
target-induced structural switching, while the AgNP-based UV channel provides a complementary, concentration-dependent signal at 405 nm, allowing cross-validation and improved reliability. The platform demonstrates a good linear detection range (0.5-20 µM), with detection limits of 0.56 µM and 0.62 µM for the fluorescence and UV modes, respectively, in serum samples. It also exhibits excellent specificity against structurally similar molecules. This dual-mode strategy offers a robust and practical alternative for therapeutic drug monitoring of MTX in complex biological environments.
Fields of Study & Subjects
Advanced biosensing and bioanalysis techniques
Biosensors and Analytical Detection
Electrochemical sensors and biosensors
Publication details
- Journal / source
- RSC Advances
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 16
- Issue
- 7
- Pages
- 6454–6463
- Publication date
- 1 January 2026
- Language
- EN