Development of an impedimetric aptasensor for Shiga-like toxin 1 using a DNA aptamer and an interdigitated microelectrode
Abstract
, underscoring its strong analytical performance. It retained 97.6% of its initial response after 25 days with five intermittent operations and showed high reproducibility, with only 3.9% standard deviation across five independently fabricated sensors. These stable performances were attributed to the strong aptamer-surface coupling enacted by covalent immobilization through a self-assembled monolayer strategy. The aptasensor further demonstrated high selectivity and practical applicability by reliably detecting the toxin protein in spiked milk samples. Overall, the developed aptasensor offer a great prospect for its practical application as a diagnostic device for rapid and reliable detection of Shiga toxin-induced gastroenteritis at point-of-care settings.
Fields of Study & Subjects
Biosensors and Analytical Detection
Escherichia coli research studies
Advanced biosensing and bioanalysis techniques
Publication details
- Journal / source
- RSC Advances
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 16
- Issue
- 5
- Pages
- 4590–4601
- Publication date
- 1 January 2026
- Language
- EN