Subunit fusion unlocks rapid in vitro maturation for slowly activating heterodimeric [FeFe]-hydrogenases

Chemical Science 2026 Open access

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Authors: Jan Jaenecke, Konstantin Bikbaev, Miriam Malagnini, Julia Bronold, Shanika Yadav, Ulf-Peter rer. nat. Apfel, Christophe Léger, James A. Birrell, Ingrid Span, Nicolas Plumeré, Martin Winkler
DOI: 10.1039/d5sc07299a
Cited by: 2
References: 49

Abstract

Subunit fusion unlocks fast maturation for slowly maturating [FeFe]-hydrogenase Dd HydAB. Linking small and large subunit stabilizes active site closure after 2Fe H uptake, accelerating H-cluster formation and activity development by 40-fold.

Fields of Study & Subjects

Metalloenzymes and iron-sulfur proteins Metal-Catalyzed Oxygenation Mechanisms Enzyme Catalysis and Immobilization

Publication details

Journal / source
Chemical Science
Publisher
Royal Society of Chemistry (RSC)
Volume
17
Issue
15
Pages
7678–7689
Publication date
1 January 2026
Language
EN