Self-releasing reactive oxygen species based on the metal-to-MOF charge transfer effect boost electrochemiluminescence

MMCT, enabling ROS generation without exogenous oxidants. This mechanism enhances the ECL signal of luminol-derived carbon dots (L-CDs) through ROS-mediated pathways. Integrating L-CDs with Zn-MOF creates a unique reaction environment that shortens the distance between L-CDs and ROS and stabilizes the L-CDs intermediate active species, thereby improving ECL signal strength and stability in a neutral environment. The system also incorporates the specific binding of ochratoxin A to its aptamer, releasing activated DNA to trigger CRISPR/Cas12a-mediated cleavage of single-stranded DNA (ssDNA) anchored to magnetic beads and dopamine (DA). After magnetic separation, DA-ssDNA is modified on the electrode surface to suppress the initial ECL response. This sensing platform offers a robust solution for detecting mycotoxins in complex matrices, with applications in food safety and environmental monitoring.