Rational design of structurally rich DNA aptamer screening libraries guided by empirical sequence trends and stable loop motifs
Abstract
UV-vis melting analysis confirmed this predicted trend in thermal stability. Additionally, quantitative polymerase chain reaction (qPCR) analysis demonstrated efficient and unbiased amplification efficiency across all three libraries. Finally, an adapted next generation sequencing (NGS) protocol enabled sequence analysis that confirmed compatibility of each library with high throughput Illumina platforms. By biasing heterogeneous DNA populations towards empirical nucleotide compositions and experimentally validated structural elements, this approach enables the design of oligonucleotide screening libraries to enrich potential target-binding motifs.
Fields of Study & Subjects
Advanced biosensing and bioanalysis techniques
DNA and Nucleic Acid Chemistry
RNA and protein synthesis mechanisms
Publication details
- Journal / source
- Journal of Materials Chemistry B
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 14
- Issue
- 11
- Pages
- 3490–3500
- Publication date
- 1 January 2026
- Language
- EN