A highly-efficient isothermal nano-detection platform coupling CRISPR/Cas technology for detection of circRNA
Abstract
-) and Cas9n. Furthermore, it combines Cas12a and immunomagnetic beads for an extra signal boost and output. After confirming its feasibility and optimizing the conditions, the detection system achieved a 1.73-fold enhancement in sensitivity, offering a linear detection range of 1 pM to 100 nM, with the limit of detection lowered to 95.1 fM. It also showed good specificity through testing against 5 biomarkers. Therefore, this detection system provides a novel strategy for the early diagnosis of TNBC and other diseases.
Fields of Study & Subjects
CRISPR and Genetic Engineering
Advanced biosensing and bioanalysis techniques
Biosensors and Analytical Detection
Publication details
- Journal / source
- The Analyst
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 151
- Issue
- 5
- Pages
- 1304–1309
- Publication date
- 1 January 2026
- Language
- EN