An “off-on” fluorescent sensor based on FRET and magnetic beads for APE1 activity detection in breast cancer cell lysates
Abstract
for purified APE1 and can detect activity from as few as 50 breast cancer cells. It reliably distinguishes APE1 levels between normal (MCF-10A) and cancerous (MCF-7, MDA-MB-231) cells, with >96% recovery in serum. This work provides a practical tool for activity-based APE1 detection in cancer research and diagnostics.
Fields of Study & Subjects
Advanced biosensing and bioanalysis techniques
Carbon and Quantum Dots Applications
Nanoplatforms for cancer theranostics
Publication details
- Journal / source
- Analytical Methods
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 18
- Issue
- 12
- Pages
- 2455–2463
- Publication date
- 1 January 2026
- Language
- EN