DNA-mediated synergistic proximity ligation for ultrasensitive electrochemiluminescence detection of microcystin-LR
Abstract
"3-DNA cooperative complex" under identical conditions. The stronger ECL intensity observed in the "4-DNA cooperative complex" confirmed the synergistic proximity ligation effect, highlighting its critical role in facilitating the DNA hybridization. The developed PLA-based ECL aptasensor demonstrated high sensitivity and selectivity and quick response and it was successfully applied to the detection of MC-LR in tap water. The novelty of this work lies in the strategic design of a dual-quencher DNA probe and the implementation of a competitive, synergistic PLA, which together enable ultrasensitive detection of small molecules like MC-LR, a challenge for traditional PLA methods.
Fields of Study & Subjects
Advanced biosensing and bioanalysis techniques
Biosensors and Analytical Detection
Aquatic Ecosystems and Phytoplankton Dynamics
Publication details
- Journal / source
- Analytical Methods
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 18
- Issue
- 15
- Pages
- 3065–3073
- Publication date
- 1 January 2026
- Language
- EN