DNAzyme–aptamer electrochemical biosensor for Pb( ii ) and Hg( ii ) determination: robust validation in food matrices and water samples
Abstract
dominated by partial signal compensation rather than frequency-resolved deconvolution. The platform also showed high selectivity against competing metal ions, same-electrode repeatability below 4%, between-batch fabrication precision below 5%, and storage stability above 92% over 28 days. Fit-for-purpose validation was performed in tap water, river water, seawater, milk, fish, and spinach samples, yielding recoveries of 91.2-108.0% and good agreement with ICP-MS. These results support the use of the present GO-AuNP/DNAzyme-aptamer architecture as a practical screening platform for trace heavy-metal monitoring in complex real samples.
Fields of Study & Subjects
Advanced biosensing and bioanalysis techniques
Electrochemical Analysis and Applications
Molecular Sensors and Ion Detection
Publication details
- Journal / source
- Analytical Methods
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 18
- Issue
- 21
- Pages
- 4444–4453
- Publication date
- 1 January 2026
- Language
- EN