An exonuclease III-assisted CHA dual-cycle amplification strategy for quencher-free fluorescent detection of miRNA

Analytical Methods 2026

View at publisher
Authors: Shi-Qian Liao, Jia-Hao Liu, Xin-Yue Liu, Yiting Liu, Chunguang Yang, Zhangrun Xu
DOI: 10.1039/d6ay00629a
Cited by: 1
References: 38

Abstract

. Furthermore, the method demonstrated excellent single-base mismatch discrimination and was successfully applied to the recovery test of miRNA-21 in human serum and the detection of miRNA-21 incancer cell lysates. Therefore, this work is expected to become a simple, sensitive and specific method for detecting miRNAs, which will contribute to the clinical detection of tumor markers.

Fields of Study & Subjects

Advanced biosensing and bioanalysis techniques MicroRNA in disease regulation DNA and Nucleic Acid Chemistry

Publication details

Journal / source
Analytical Methods
Publisher
Royal Society of Chemistry (RSC)
Volume
18
Issue
23
Pages
4917–4926
Publication date
1 January 2026
Language
EN