Development of a bioluminescent enzyme immunoassay for glycocholic acid based on a single-chain variable fragment-nanoluciferase fusion

Analytical Methods 2026

View at publisher
Authors: Yunping Mu, Huajian Cai, Xindan Zhang, Mingxia Lin, Junhao Zeng, Yiyun Wang, Ziming Wu, Suqing Zhao, Xiping Cui
DOI: 10.1039/d6ay00731g
Cited by: 1
References: 33

Abstract

. The BLEIA provided not only high selectivity for GCA analogues but also the satisfactory recoveries ranging from 99.26% to 117.18%. Thus, these results indicated that scFv-Nluc was an effective immunodetection tool for developing BLEIA, which has significant potential for GCA analysis.

Fields of Study & Subjects

bioluminescence and chemiluminescence research Advanced biosensing and bioanalysis techniques Glycosylation and Glycoproteins Research

Publication details

Journal / source
Analytical Methods
Publisher
Royal Society of Chemistry (RSC)
Volume
18
Issue
23
Pages
4819–4825
Publication date
1 January 2026
Language
EN