Comparative analysis of alkyne- and desthiobiotinylated photoaffinity probes for chemotranscriptomic profiling

RSC Chemical Biology 2026 Open access

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Authors: Daphne A. L. van den Homberg, Georgia Poulladofonou, Aurelia Dekens, Willem A. Velema
DOI: 10.1039/d6cb00030d
Cited by: 0
References: 1

Abstract

transcription/translation and RT-qPCR. We also observed high unspecific interactions due to proposed weak and non-specific binding of the desthiobiotin moiety to RNA analyzed by dot blots and RT-qPCR. Finally, transcriptome-wide sequencing confirmed the unselective interaction of desthiobiotin. These findings suggest that desthiobiotin is an inefficient enrichment handle for the design of photoaffinity probes, resulting in many off-target interactions.

Fields of Study & Subjects

Biotin and Related Studies Click Chemistry and Applications RNA and protein synthesis mechanisms

Publication details

Journal / source
RSC Chemical Biology
Volume
7
Issue
4
Pages
641–648
Publication date
1 January 2026
Language
EN