Switchable glycan probe enables pH-dependent activity modulation of UDP-glucose: glycoprotein glucosyltransferase through a responsive aglycone

Chemical Communications 2026

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Authors: Mitsuaki Hirose, Y Suzuki, Rentaro Miyuki, Kosuke Oki, K Sato, Akihiro Yokoyama, Kiichiro Totani
DOI: 10.1039/d6cc02099b
Cited by: 1
References: 36

Abstract

UGGT1 assays revealed that the switchable glycan probe exhibits glucose transfer activity in the order of pH 6.0 > pH 8.5, while a unresponsive control probe showed no pH-dependent change in activity. Furthermore, UGGT1 activity could be dynamically switched during the reaction by changing the pH. These results demonstrate that aglycone structural populations can actively regulate UGGT1 activity, providing the first example of controlling UGGT1-mediated glucose transfer using a single, environmentally switchable glycan probe. This work establishes an innovative molecular design strategy, opening a conceptual framework to probe and manipulate ER glycoprotein quality control mechanism using chemically programmable glycan probes.

Fields of Study & Subjects

Glycosylation and Glycoproteins Research Carbohydrate Chemistry and Synthesis Click Chemistry and Applications

Publication details

Journal / source
Chemical Communications
Publisher
Royal Society of Chemistry (RSC)
Volume
62
Issue
60
Pages
14992–14995
Publication date
1 January 2026
Language
EN