Intensified lentiviral vector perfusion bioprocessing with a spiral inertial microfluidic cell retention device
Abstract
transducing units (TU) per mL. These production titers are competitive with other bioprocessing approaches in industry and academia. The highest cell-specific productivity (over 50 TU per cell per day) and cell-specific yields of our study (over 80 TU per cell) were achieved when using spiral device-mediated perfusion bioprocessing to cultivate cells and then transferring the cells to a shake flask environment with daily media replacement to generate lentiviral vectors.
Fields of Study & Subjects
3D Printing in Biomedical Research
Virus-based gene therapy research
Microbial Inactivation Methods
Publication details
- Journal / source
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 26
- Issue
- 7
- Pages
- 2368–2379
- Publication date
- 1 January 2026
- Language
- EN