Pixel-based AFM-IR uncovers nanoscale lipid remodeling in MPNST cells
Abstract
MPNST cells under CBD and irradiation, including pronounced spatial reorganization that was not evident from spectral intensities alone. Importantly, the AFM-IR-derived spatial metrics were independently validated by fluorescence lipid droplet staining, demonstrating similar trends between nanoscale infrared measurements and cellular lipid abundance. In parallel, AFM-IR analysis of the Amide I and II regions uncovered CBD-dependent modulation of protein secondary structure, highlighting differential responses between normal and malignant cells. Overall, this study establishes a transferable, pixel-based AFM-IR analysis strategy for nanoscale biochemical semi-quantification and demonstrates its utility in resolving lipid organization and remodeling in complex biological systems.
Fields of Study & Subjects
Lipid Membrane Structure and Behavior
Spectroscopy Techniques in Biomedical and Chemical Research
Spectroscopy and Quantum Chemical Studies
Publication details
- Journal / source
- Nanoscale
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 18
- Issue
- 22
- Pages
- 11938–11949
- Publication date
- 1 January 2026
- Language
- EN