Heterologous expression and in vivo characterization of the longipeptin biosynthetic gene cluster

Organic & Biomolecular Chemistry 2026

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Authors: Yuwei Duan, Menghan Shi, Wei Sun, Youming Zhang, Xiaoying Bian, Guannan Zhong
DOI: 10.1039/d6ob00049e
Cited by: 1
References: 48

Abstract

-methylation to afford trivalent sulfonium. All the three secondary modifications occur post-macrolactamization, with the LopF-catalyzed crosslinking serving as an essential prerequisite for LopH-mediated methylation. Our results establish a framework for investigating P450-catalyzed lasso peptide crosslinking, represent the first functional characterization of a DUF6919 family protein and reveal distinct strategies for sulfonium installation in RiPPs.

Fields of Study & Subjects

Microbial Natural Products and Biosynthesis RNA and protein synthesis mechanisms Antimicrobial Peptides and Activities

Publication details

Journal / source
Organic & Biomolecular Chemistry
Publisher
Royal Society of Chemistry (RSC)
Volume
24
Issue
11
Pages
2307–2315
Publication date
1 January 2026
Language
EN