Discovery of four novel furanosteroids and a new 4-methylprogesteroid from demethoxyviridin pathway-disrupted mutants of Nodulisporium sp. through cultivation time extension
Abstract
. Among them, five compounds are novel, including four furanosteroids (1-4) and a 4-methylprogesteroid (5), which may serve as a biosynthetic precursor of furanosteroids. Intriguingly, structural analysis of these new compounds enabled us to propose functional hypotheses for the previously uncharacterized enzymes: VidR is potentially involved in C-7 oxidation, and VidO likely acts downstream of VidR. The cytotoxicity assay revealed that compound 2 displayed remarkable cytotoxic effects on human PANC-1 cells. This work demonstrated that combining biosynthetic gene disruption with extended fermentation is an effective approach to generate novel analogues by redirecting biosynthetic flux.
Fields of Study & Subjects
Microbial Natural Products and Biosynthesis
Steroid Chemistry and Biochemistry
Plant biochemistry and biosynthesis
Publication details
- Journal / source
- Organic & Biomolecular Chemistry
- Volume
- 24
- Issue
- 10
- Pages
- 2079–2086
- Publication date
- 1 January 2026
- Language
- EN