Acetohydroxamic acid-assisted peptide hydrazide ligation for chemical protein synthesis
Abstract
-acyl hydroxamates, which undergo efficient and direct ligation with N-terminal cysteine-containing peptides. This thiol-additive-free approach is compatible with one-pot ligation-desulfurization and ligation-oxidative folding. The utility of this strategy is demonstrated by the expedient synthesis of site-specifically lactylated histone H3 (H3K18la) and the disulfide-rich neurotoxin Calciseptine. Our study expands the repertoire of hydrazide-based ligation and provides a practical, operationally straightforward tool to accelerate chemical protein synthesis.
Fields of Study & Subjects
Chemical Synthesis and Analysis
Click Chemistry and Applications
Peptidase Inhibition and Analysis
Publication details
- Journal / source
- Organic & Biomolecular Chemistry
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 24
- Issue
- 10
- Pages
- 2061–2068
- Publication date
- 1 January 2026
- Language
- EN