Nanoflower-shaped cobalt-metal–organic framework as an oxidase-like nanozyme and 1,2-diaminobenzene as a catalytic substrate for the innovative signal “off-on” aptamer sensing of prostate-specific antigen
Abstract
. However, when the prepared Co-MOF nanozyme binds to PSA-specific aptamers, its enzyme-like activity becomes inhibited due to the blockage of active sites by the aptamer, leading to the disappearance of the DAP current that corresponds to the signal "off" state. Notably, in the presence of PSA, the catalytic activity of the Co-MOF is restored as the specific binding between Apt and PSA causes Apt to detach from the Co-MOF surface, resulting in the recovery of the DAP current and switching the signal to the "on" state. After the optimization of key experimental parameters, the proposed "off-on" nanozyme-based electrochemical aptasensor demonstrates excellent PSA detection performance. Moreover, this platform may provide a novel, simple, and reliable strategy for detecting a wide range of biomarkers by simply replacing the corresponding aptamer.
Fields of Study & Subjects
Advanced Nanomaterials in Catalysis
Advanced biosensing and bioanalysis techniques
Nanocluster Synthesis and Applications
Publication details
- Journal / source
- RSC Advances
- Publisher
- Royal Society of Chemistry (RSC)
- Volume
- 16
- Issue
- 26
- Pages
- 23288–23294
- Publication date
- 1 January 2026
- Language
- EN