Target deconvolution of a selective HepG2 cytotoxic hit identifies GSTP1 and TRAP1 as candidate molecular targets

RSC Advances 2026 Open access

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Authors: Belal Omar Al-Najjar, M. Helal, Fadi G. Saqallah, B. Bandy
DOI: 10.1039/d6ra04601k
Cited by: 1
References: 23

Abstract

toxicological profiling classified HTS00019 as oral toxicity class 4 and flagged potential hepatotoxicity, mutagenicity, immunotoxicity, and carcinogenicity liabilities, while p53- and ATAD5-related stress pathway predictions were inactive. Overall, HTS00019 was identified as a selective HepG2 cytotoxic hit, with GSTP1 and TRAP1 emerging as computationally prioritized candidate targets. However, the absence of biochemical target validation and the predicted toxicological liabilities indicate that HTS00019 remains an early-stage hit requiring mechanistic confirmation and structural optimization.

Fields of Study & Subjects

Protein Kinase Regulation and GTPase Signaling Glutathione Transferases and Polymorphisms Enzyme function and inhibition

Publication details

Journal / source
RSC Advances
Publisher
Royal Society of Chemistry (RSC)
Volume
16
Issue
37
Pages
39646–39661
Publication date
1 January 2026
Language
EN