Traceless protein semisynthesis in cells using the promiscuous ultra-fast split intein NrdJ-1

Chemical Science 2026 Open access

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Authors: Xuanjia Ye, Joshua Sokol, Christian Kofoed, Anushka Dheer, Juner Zhang, Tom W. Muir
DOI: 10.1039/d6sc01808d
Cited by: 1
References: 43

Abstract

and in living cells, as demonstrated by the traceless generation of semisynthetic chromatin harboring various post-translational modifications. These studies highlight the utility of NrdJ-1 for traceless protein editing and suggest it is a superior choice for many intracellular biochemical applications.

Fields of Study & Subjects

RNA and protein synthesis mechanisms Cell Adhesion Molecules Research Protein Degradation and Inhibitors

Publication details

Journal / source
Chemical Science
Publisher
Royal Society of Chemistry (RSC)
Volume
17
Issue
22
Pages
11157–11165
Publication date
1 January 2026
Language
EN